abstract：:Hematopoietic cells have the potential for providing benefit in a variety of clinical settings. These include cells for support of patients undergoing high-dose chemotherapy, as a target for replacement gene therapy, and as a source of cells for immunotherapy. The limitation to many of these applications has been the ...

journal_title：Experimental hematology

authors： McNiece I,Briddell R

更新日期：2001-01-01 00:00:00

abstract：:The rae28 gene (rae28) is a murine homologue of the Drosophila polyhomeotic gene, which is a member of the Polycomb-group genes. In this study, we examined the role of rae28 in lymphocyte development. Because homozygous rae28-deficient (rae28-/-) mice died in the perinatal period, we examined lymphocyte development by...

journal_title：Experimental hematology

authors： Tokimasa S,Ohta H,Sawada A,Matsuda Y,Kim JY,Nishiguchi S,Hara J,Takihara Y

更新日期：2001-01-01 00:00:00

abstract：:More than 90% of patients with severe congenital neutropenia (SCN) respond to granulocyte colony-stimulating factor (G-CSF) therapy. The basis for the refractory state in the remaining patients is unknown. To address this issue, we studied a child with SCN who was totally unresponsive to G-CSF and had a novel point mu...

journal_title：Experimental hematology

authors： Dror Y,Ward AC,Touw IP,Freedman MH

更新日期：2000-12-01 00:00:00

abstract：:Functional recovery of the immune system is critical for long-term survival in hematopoietic stem cell transplant recipients. In this study, two donor-recipient allogeneic transplant settings (haploidentical and fully mismatched) are used to investigate the functional activity of donor-derived B and T cells in animals...

journal_title：Experimental hematology

authors： Bowers E,Tamaki S,Coward A,Kaneshima H,Chao CC

更新日期：2000-12-01 00:00:00

abstract：:Patients who undergo autologous bone marrow transplantation for acute leukemia are at high risk for relapse. We have evaluated the feasibility of administering cell-mediated immunotherapy with family-related haploidentical lymphocytes following autologous bone marrow transplantation in order to evoke a graft-vs-leukem...

journal_title：Experimental hematology

authors： Nagler A,Ackerstein A,Or R,Naparstek E,Slavin S

更新日期：2000-11-01 00:00:00

abstract：OBJECTIVE:Hemopoiesis is regulated by cytokines with positive or negative effects on proliferation of lineage-committed or multipotent hemopoietic stem cells. We have investigated the roles of interleukin-6 and other gp130-dependent ligands on the proliferation of macrophage-lineage hemopoietic progenitor cells. METHO...

journal_title：Experimental hematology

authors： Clutterbuck R,Powles R,Millar J,Catovsky D

更新日期：2000-10-01 00:00:00

abstract：OBJECTIVE:Mobilized peripheral blood (PB) progenitors are increasingly used in autologous and allogeneic transplantation. However, the short- and long-term engraftment potential of mobilized PB or bone marrow (BM) has not been directly compared. Although several studies showed that BM-derived Lin(-)CD34(-) cells contai...

journal_title：Experimental hematology

authors： Verfaillie CM,Almeida-Porada G,Wissink S,Zanjani ED

更新日期：2000-09-01 00:00:00

abstract：OBJECTIVE:The purpose of this study was to investigate the effects of interleukin-2 (IL-2) gene-transduced hematopoietic progenitor cells or cytotoxic function and systemic toxicity following syngeneic bone marrow transplantation. MATERIAL AND METHODS:Marrow of 5-fluorouracil pretreated donor mice were transfected wit...

journal_title：Experimental hematology

authors： Kühr T,Bechter O,Dirnhofer S,Geley S,Gächter A,Pall G,Url M,Dietrich H,Oberaigner P,Klima G,Eisterer W,Hilbe W,Lukas P,Thaler J

更新日期：2000-08-01 00:00:00

abstract：OBJECTIVE:Excessive apoptosis may have a role in the ineffective hematopoiesis and cytopenias observed in myelodysplastic syndromes. The goals of this study were 1) to quantify apoptosis in patients with "early stage" myelodysplasia [including patients with refractory anemia (RA), RA with ringed sideroblasts (RARS), RA...

journal_title：Experimental hematology

authors： Bouscary D,Chen YL,Guesnu M,Picard F,Viguier F,Lacombe C,Dreyfus F,Fontenay-Roupie M

更新日期：2000-07-01 00:00:00

abstract：:We investigated whether gene transfer into hematopoietic cells could be achieved by direct injection of retroviral vector supernatant into the bone marrow space of newborn sheep. Six sheep (5 weeks old) were injected bilaterally with either 1 mL of G1nBgSvNa8.1 vector supernatant (titer: 1 x 10(7)) in each hip (n = 5)...

journal_title：Experimental hematology

authors： Porada CD,Tran ND,Zhao Y,Anderson WF,Zanjani ED

更新日期：2000-06-01 00:00:00

abstract：OBJECTIVE:To test gibbon ape leukemia virus (GALV) pseudotype vector transduction of marrow subpopulations that contribute to hematopoietic reconstitution in vivo. MATERIALS AND METHODS:Autologous CD34(+) Lin(-), CD34(+) Lin(+), and CD34(-) Lin(-) marrow cells, transduced by coculture with PG13/LN, PG13/LNX, and PG13/...

journal_title：Experimental hematology

authors： Andrews RG,Peterson LJ,Morris J,Potter J,Heyward S,Gough M,Bryant E,Kiem HP

更新日期：2000-05-01 00:00:00

abstract：OBJECTIVE:Osteoblasts play an important role in regulating hematopoiesis in the bone marrow. Here we show that U2-OS, a widely used osteoblastic cell line derived from an osteosarcoma, has the capacity to support proliferation of human hematopoietic progenitor cells in vitro. In this study, U2-OS cells are characterize...

journal_title：Experimental hematology

authors： Nelissen JM,Torensma R,Pluyter M,Adema GJ,Raymakers RA,van Kooyk Y,Figdor CG

更新日期：2000-04-01 00:00:00

abstract：OBJECTIVE:TGF-beta1 is a powerful inhibitor of erythropoiesis. However, its mechanisms of action are not fully elucidated yet at the cellular level. In this work we have studied the effects of TGF-beta on UT-7 cell survival, proliferation and differentiation. MATERIALS AND METHODS:UT-7 cell line is strictly dependent ...

journal_title：Experimental hematology

authors： Zermati Y,Varet B,Hermine O

更新日期：2000-03-01 00:00:00

abstract：OBJECTIVE:We previously reported on the heterogeneity of bone marrow stromal cell function in supporting hematopoietic cell proliferation and differentiation in vitro among refractory anemia (RA) of myelodysplastic syndrome (MDS) patients. Interestingly, stromal cells from some MDS RA patients induced an apoptotic chan...

journal_title：Experimental hematology

authors： Aizawa S,Hiramoto M,Hoshi H,Toyama K,Shima D,Handa H

更新日期：2000-02-01 00:00:00

abstract：OBJECTIVE:The purpose of this study was to evaluate the repopulating properties of bone marrow (BM) from mice irradiated during embryonic and adult stages of development. MATERIALS AND METHODS:Four-day-old embryos, 17-day-old fetuses, and 12-week-old mice were irradiated with of 1 or 3 Gy of x-rays. At 3 and 9 months ...

journal_title：Experimental hematology

authors： Grande T,Varas F,Bueren JA

更新日期：2000-01-01 00:00:00

abstract：:Granulocyte colony-stimulating factor (G-CSF) has proven effective in the prophylaxis of chemotherapy-induced neutropenia and as a mobilizer of peripheral blood progenitor cells. The longevity of G-CSF action is limited by its removal from the body by two mechanisms. The first is thought to be mediated via receptors (...

journal_title：Experimental hematology

authors： Molineux G,Kinstler O,Briddell B,Hartley C,McElroy P,Kerzic P,Sutherland W,Stoney G,Kern B,Fletcher FA,Cohen A,Korach E,Ulich T,McNiece I,Lockbaum P,Miller-Messana MA,Gardner S,Hunt T,Schwab G

更新日期：1999-12-01 00:00:00

abstract：:Several prospective randomized trials in acute myelocytic leukemia (AML) documented a lower relapse rate with autologous bone marrow transplantation (ABMT) than with conventional chemotherapy. However, they also identified some transplant difficulties, such as failure to collect sufficient numbers of stem cells, slow ...

journal_title：Experimental hematology

authors： Gorin NC,Labopin M,Laporte JP,Douay L,Lopez M,Lesage S,Fouillard L,Isnard F,Jouet JP,Bellal N,Perot C,Van Den Akker J,Bauters F,Najman A

更新日期：1999-12-01 00:00:00

abstract：:We previously demonstrated that lysis of tumor cells that express Hsp70, the highly stress-inducible member of the HSP70 family, on their plasma membrane is mediated by natural killer (NK) cells. Here, we studied the effects of different proteins of the HSP70 family in combination with interleukin 2 (IL-2) on the prol...

journal_title：Experimental hematology

authors： Multhoff G,Mizzen L,Winchester CC,Milner CM,Wenk S,Eissner G,Kampinga HH,Laumbacher B,Johnson J

更新日期：1999-11-01 00:00:00

abstract：:Cyclooxygenase (COX) plays a key regulatory role in prostaglandin synthesis. COX-2 is inducible and is the major isoform of inflammatory cells. COX-2-deficient mice were shown to have normal basal hematopoiesis and hematology. We hypothesized that COX-2 induction plays a role in the recovery phase of 5-fluorouracil (5...

journal_title：Experimental hematology

authors： Lorenz M,Slaughter HS,Wescott DM,Carter SI,Schnyder B,Dinchuk JE,Car BD

更新日期：1999-10-01 00:00:00

abstract：:Improvement in diagnostic cytogenetic techniques has led to the recognition of an increasing number of leukemia-associated chromosomal translocations and inversions. These genetic lesions frequently are associated with the disruption of putative transcription factors and the production of hybrid transcripts that are i...

journal_title：Experimental hematology

authors： Spencer A,Granter N

更新日期：1999-09-01 00:00:00

abstract：:The osteoclast is a hematopoietic cell derived from CFU-GM and branches from the monocyte-macrophage lineage early during the differentiation process. The marrow microenvironment appears critical for osteoclast formation due to production of RANK ligand, a recently described osteoclast differentiation factor, by marro...

journal_title：Experimental hematology

authors： Roodman GD

更新日期：1999-08-01 00:00:00

abstract：OBJECTIVE:The induction of transplantation tolerance across xenogeneic barriers by bone marrow transplantation holds great promise, but engraftment of xenogeneic stem cells has been difficult to achieve. Part of this difficulty is due to species-specific differences in regulatory cytokines and elements of the stromal m...

journal_title：Experimental hematology

authors： Emery DW,Holley K,Sachs DH

更新日期：1999-08-01 00:00:00

abstract：:Fabry disease is a lysosomal storage disorder that is due to a deficiency in alpha-galactosidase A (alpha-gal A). Previously we have shown that a recombinant retrovirus synthesized for the transfer of the human alpha-gal A coding sequence was able to engineer enzymatic correction of the hydrolase deficiency in fibrobl...

journal_title：Experimental hematology

authors： Takenaka T,Hendrickson CS,Tworek DM,Tudor M,Schiffmann R,Brady RO,Medin JA

更新日期：1999-07-01 00:00:00

abstract：:Murine low-density bone marrow cells sorted from the blast cell window on the basis of high rhodamine-123 retention (Rh-bright), are highly enriched in histamine-, IL-4-, and IL-6-producing cells. We established by in situ hybridization that up to 50% of this population (around 0.25% of the whole bone marrow) coexpres...

journal_title：Experimental hematology

authors： Schneider E,Lemoine FM,Breton-Gorius J,Machavoine F,Arnould A,Cramer EM,Guichard J,Michel D

更新日期：1999-06-01 00:00:00

abstract：:Arsenic trioxide (As2O3) has been demonstrated to be effective for the treatment of acute promyelocytic leukemia (APL) and to inhibit proliferation and produce apoptosis in the APL cell line NB4. To determine if As2O3 might be useful for the treatment of other lineages, we investigated the effects of As2O3 on viabilit...

journal_title：Experimental hematology

authors： Lu M,Levin J,Sulpice E,Sequeira-Le Grand A,Alemany M,Caen JP,Han ZC

更新日期：1999-05-01 00:00:00

abstract：:A peptide sequence was identified by phage display technology that could be used as an alternative to chymopapain for the release of hematopoietic progenitor cells captured by anti-CD34 monoclonal antibodies. This was achieved by affinity selection screening (biopanning) of a random hexapeptide sequence phage display ...

journal_title：Experimental hematology

authors： Tseng-Law J,Szalay P,Guillermo R,Kobori J,Van Epps D,Schneidkraut MJ,Deans R

更新日期：1999-05-01 00:00:00

abstract：:We have developed a prospective approach to identify the generation of erythroid cells derived from cultured peripheral blood mononuclear cells (PBMC) by monitoring the expression of the cell surface protein CD48. Unpurified populations of PBMC obtained from the buffy coats of normal volunteers were grown in suspensio...

journal_title：Experimental hematology

authors： Miller JL,Njoroge JM,Gubin AN,Rodgers GP

更新日期：1999-04-01 00:00:00

abstract：:We established a clonal cell culture system for human natural killer (NK) cells from fetal thymocytes. Thymocytes of 16 to 22 gestational weeks were cultured in methylcellulose in the presence of interleukin (IL)-7, IL-15, and steel factor (SF). After 14 days in incubation, large, diffuse colonies consisting of small ...

journal_title：Experimental hematology

authors： Sato T,Laver JH,Aiba Y,Ogawa M

更新日期：1999-04-01 00:00:00

abstract：:Megakaryocytic differentiation of progenitor cells was investigated in nine patients with low-risk myelodysplastic syndromes (MDS) (eight refractor anemia [RA] and one RA with ringed sideroblasts [RARS] and five patients with high-risk MDS (two RA with excess of blasts [RAEB] and three RAEB in transformation [RAEB-T])...

journal_title：Experimental hematology

authors： Hofmann WK,Kalina U,Wagner S,Seipelt G,Ries C,Hoelzer D,Ottmann OG

更新日期：1999-03-01 00:00:00

abstract：:We analyzed the accessory function of malignant B cells from non-Hodgkin's lymphomas (NHLs). Among the 70 samples of malignant B cells included, four patterns of expression of the costimulatory molecules CD80 and CD86 were distinguished (+/+, +/-, -/+ and -/-). In two-thirds of the cases, CD80, CD86, or both were expr...

journal_title：Experimental hematology

authors： Chaperot L,Plumas J,Jacob MC,Bost F,Molens JP,Sotto JJ,Bensa JC

更新日期：1999-03-01 00:00:00

abstract：:We compared the kinetic recovery of lymphocytes and their subsets in two groups of patients submitted to allogeneic peripheral blood progenitor cell transplantation (allo-PBT): those receiving lymphocyte-depleted leukaphereses by positive selection of CD34+ cells (group 1, n = 18) and those receiving unmanipulated leu...

journal_title：Experimental hematology

authors： Martínez C,Urbano-Ispizua A,Rozman C,Marín P,Rovira M,Sierra J,Montfort N,Carreras E,Montserrat E

更新日期：1999-03-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNFalpha) is thought to contribute to the blunted erythropoietin (Epo) production in inflammatory diseases. The present study was carried out to find out as to whether the 55 kD (TNF-RI) or the 75 kD (TNF-RII) receptor is responsible for the TNFalpha-induced inhibition of hepatic Epo synth...

journal_title：Experimental hematology

authors： Jelkmann W,Hellwig-Buergel T

更新日期：1999-02-01 00:00:00

abstract：:The mechanism of localization, migration, and regulation of hematopoiesis at different stages of ontogeny is not well understood, but may relate to the specific adhesive interactions between hematopoietic stem cells and their microenvironment at different ontogenic stages. We studied the expression of cell adhesion mo...

journal_title：Experimental hematology

authors： Roy V,Verfaillie CM

更新日期：1999-02-01 00:00:00

abstract：:Inconsistent results characterized N-acetyl-Ser-Asp-Lys-Pro (AcSDKP or Goralatide) effects upon hematologic proliferation, possibly because its circadian organization had been overlooked. We investigated the circadian changes in AcSDKP disposition in plasma and in bone marrow during continuous infusion and AcSDKP effe...

journal_title：Experimental hematology

authors： Li XM,Ezan E,Bindoula G,Soulard C,Liu XH,Deschamps de Paillette E,Lévi F

更新日期：1999-01-01 00:00:00

abstract：:A single injection of > or =10 microg/kg PEG-rHuMGDF in mice causes a dose-dependent increase in circulating platelets beginning on day 3 and peaking on days 5-6. The mean platelet volume and platelet distribution width at doses > or =100 microg/kg initially increase in a dose-dependent fashion and later decrease. How...

journal_title：Experimental hematology

authors： Ulich TR,del Castillo J,Senaldi G,Cheung E,Roskos L,Young J,Molineux G,Guo J,Schoemperlen J,Munyakazi L,Murphy-Filkins R,Tarpley JE,Toombs CF,Kaufman S,Yin S,Nelson AG,Nichol JL,Sheridan WP

更新日期：1999-01-01 00:00:00

abstract：:Normal human peripheral blood mononuclear cells (MNCs), particularly T lymphocytes (T cells), are a rich source of granulocyte-macrophage colony-stimulating factor (GM-CSF). Glucocorticoids are known to inhibit GM-CSF production in in vitro cultures of a human fibroblast cell line and in normal human blood monocytes a...

journal_title：Experimental hematology

authors： Chikkappa G,Lansing LS,Chu RC,Pasquale D

更新日期：1998-12-01 00:00:00

abstract：:Sphingosine, a sphingolipid breakdown product, has been proposed as an apoptosis-inducing agent. In this study, we examined the effect of sphingosine in bcl-2-overexpressing cells compared with cells that do not express the bcl-2 gene. The human erythroleukemic cell line TF1, which lacks bcl-2 expression, was easily i...

journal_title：Experimental hematology

authors： Isogai C,Murate T,Tamiya-Koizumi K,Yoshida S,Ito T,Nagai H,Kinoshita T,Kagami Y,Hotta T,Hamaguchi M,Saito H

更新日期：1998-11-01 00:00:00

abstract：:CD34 is a cell-surface glycoprotein expressed in a developmental, stage-specific manner by bone marrow stem and progenitor cells. In this study we explored a possible role for c-Myb in CD34 regulation during developmental hematopoiesis. The results indicate that c-Myb can induce CD34 expression in hematopoietic and no...

journal_title：Experimental hematology

authors： Krause DS,Mucenski ML,Lawler AM,May WS

更新日期：1998-10-01 00:00:00

abstract：:In vitro exposure of murine hematopoietic stem cells (HSCs) to cell cycle-inducing cytokines has been shown to result in a defect in the ability of these cells to engraft. We used a porcine microvascular endothelial cell (PMVEC) line in conjunction with exogenous interleukin (IL)-3, IL-6, granulocyte-macrophage colony...

journal_title：Experimental hematology

authors： Brandt JE,Galy AH,Luens KM,Travis M,Young J,Tong J,Chen S,Davis TA,Lee KP,Chen BP,Tushinski R,Hoffman R

更新日期：1998-09-01 00:00:00

abstract：:The fate of hematopoietic progenitor cells (HPCs) in the bone marrow (BM) microenvironment is determined by two different interactions: 1) they adhere (via integrins) to both extracellular matrix molecules and BM stromal cells; and 2) stromal cells produce cytokines that influence their survival, proliferation, differ...

journal_title：Experimental hematology

authors： Weimar IS,Miranda N,Muller EJ,Hekman A,Kerst JM,de Gast GC,Gerritsen WR

更新日期：1998-08-01 00:00:00